Whole Plasmid Sequencing Guidelines and Fees

sequencing table

Instructions:

  1. Register with GDEC and FOM if you aren't already registered.
  2. Set up sequencing reactions according to the guidelines above.  Samples should be in 8-well strips (<=24 samples) or a PCR plate (>24 samples).  Samples should be setup by COLUMN, so that samples 1, 2, 3 correspond to 1A, 1B, 1C.
  3. Label your sample submission:  Date (YYMMDD) - User Name.  The date is the submission date to GDEC.  The user name is the name of the person whose account we are using.  The data will be sent to that person.
  4. Drop off samples at the GDEC lab (BRC 215).  Please let us know if any of your samples are nonstandard (low concentration, etc.).  This helps us prepare the library accordingly.
  5. Turnaround is 2-3 days, generally.  We will announce regular service timelines soon.
  6. Data (fastqs, consensus fasta, and read length histogram) will be send to the user via a Google Drive link.  This data will be deleted after 3 weeks, so please download your files for long term storage.

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